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Zimmer, S. Mirrett, L. Reller, M. Weinstein, J. Hindler, R. Carey, S. McAllister, D. Bruckner, J. Johnston, K. An additional isolates of S. There were no very major or major errors. Aim of the study: Rapid identification and antibacterial susceptibility testing of micro-organisms isolated in blood cultures BC can be valuable for clinical management of sepsis. In the present study, we evaluated the performance of a scheme based on direct identification and susceptibility testing of gram-positive cocci by direct inoculation of specially treated fluid collected from positive Bactec culture bottles into BD Phoenix Automated Microbiology System PHX system; BD.
Results were compared to those obtained by usual standard procedures. Materials and methods: positive monomicrobic blood cultures showing gram-positive cocci Streptococcus spp. After discarding the supernatant, the bacterial layer was resuspended and then inoculated dropwise into a PHX system ID broth in order to obtain a suspension matching a McFarland 0.
The remaining of the panels setup and loading was performed according to the manufacturer's instructions. Among the antibiotic-isolate combinations of antimicrobial susceptibility tested, The number of very major, major and minor error was 4 0. Conclusion: For gram-positive cocci, the rapid direct method combining BD Phoenix System and Bactec cultures does not provide acceptable bacterial identification, but the susceptibility results obtained more rapidly are accurate for routine use and can be valuable in the clinical management of sepsis.
Methods: We evaluated clinical isolates 1 per patient with different phenotypes of resistance to quinolones, including E. Enterobacteria were selected for including consecutive isolates with the following phenotypes CLSI definitions : Group 1 54 E. NAL was evaluated only for enterobacteria.
