Official websites use. Share sensitive information only on official, secure websites. Correspondence , U. However, its risk for prostate cancer PCa is unexplored. In our tertiary referral center, type IIIb accounted for However, this classification does not have implications for differential treatment [ 4 ], or does the presence of leukocytes in prostatic secretions correlate with the severity of symptoms [ 5 ] or persist in the same patient.
Evidence suggests that prostatitis and prostate cancer PCa are closely related, but the role of the chronic inflammatory microenvironment and immune mediators in the development of PCa are not yet understood in detail [ 12 , 13 , 14 , 15 ].
Frequent promoter methylation and abnormal expression of ESRs are often detected in PCa and correlate with tumor progression [ 26 ]. For example, microRNAs transferred by exosomes can lead to altered gene expression in the acceptor cells and cause functional changes [ 31 ]. All patients and healthy probands were informed about the study and provided written consent.
All study methodologies conformed to the standards set by the Declaration of Helsinki. Min, minimum; Max, maximum; Conc. Isolated exosomes were verified using transmission electron microscopy TEM. The protein samples from blood serum exosomes were also analyzed for select factors known to be upregulated in PCa: PSA, fatty acid synthase, and survivin.
Protein samples isolated from PPM urine exosomes possessed very low concentrations and were insufficient for western blot analyses. The membranes were washed four times with PBS containing 0. After four washes with PBS 0. S1 using image studio lite version 5. Quantitative PCR was performed in duplicate. This array comprises 84 human microRNAs known to be crucially involved in PCa development and progression. Quality checks were also performed, with all samples passing PCR array reproducibility and RT efficiency.
